Last edited by Keran
Wednesday, July 22, 2020 | History

2 edition of Studies of methanogenic bacteria in sludge found in the catalog.

Studies of methanogenic bacteria in sludge

P. H. Smith

Studies of methanogenic bacteria in sludge

by P. H. Smith

  • 47 Want to read
  • 22 Currently reading

Published by Environmental Protection Agency, Office of Research and Development, Municipal Environmental Research Laboratory, for sale by the National Technical Information Service in Cincinnati, Ohio, Springfield, Va .
Written in English

    Subjects:
  • Anaerobic bacteria,
  • Sewage sludge

  • Edition Notes

    Statementby P. H. Smith
    SeriesEnvironmental protection technology series ; EPA-600/2-80-093
    ContributionsMunicipal Environmental Research Laboratory, Florida. University, Gainesville
    The Physical Object
    Paginationxii, 100 p. :
    Number of Pages100
    ID Numbers
    Open LibraryOL13564585M

    1. Essential to the study of methanogens is a mechanism for scrubbing traces of oxygen from commercially available compressed gases. Figure presents a system for routing N 2, or mixtures of 80% N 2 and 20% CO 2 (N 2:CO 2) or 80% H 2 and 20% CO 2 (H 2:CO 2) through a heated cylinder (Fig. (1)) that contains reduced-copper filings or. The methanogenic sludge was cultivated in an upflow anaerobic sludge bed (UASB) reactor fed with propionate (35 mM) as the sole substrate for a period of 80 days. Propionate degradation was shown to be severely inhibited by the addition of 50 mM acetate to the influent of the UASB reactor.

      The compositions of methanogenic communities present in anaerobic reactors have been studied mostly for laboratory-scale digesters, including up-flow anaerobic sludge blanket reactor granules and mixture reactors treating municipal solid waste (MSW) or wastewater sludge (18, 20). Methanogenic diversity in manure-treating biogas plants has been. The objective of this study was to examine the structure-toxicity relationship of N-substituted aromatic compounds to acetoclastic methanogenic bacteria. The toxicity was assayed to serum flasks by measuring methane production in granular sludge.

    Morphological and Biochemical characters of acetogenic and methanogenic bacteria was determined using gram staining and IMViC, triple sugar ion, lipid hydrolysis, starch hydrolysis and mannitol test. Growth kinetics Growth kinetics of acetogenic and methanogenic bacteria was studied under different pH of , , , , and   Anaerobic digestion (AD) of organic municipal solid waste (OMSW) is considered as a key element in sustainable municipal waste management due to its benefits for energy, environment, and economy. This process reduces emission of greenhouse gases, generates renewable natural gas, and produces fertilizers and soil amendments. Due to its advantages over other treatment methods and .


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Studies of methanogenic bacteria in sludge by P. H. Smith Download PDF EPUB FB2

Get this from a library. Studies of methanogenic bacteria in sludge. [P H Smith; Municipal Environmental Research Laboratory.; University of Florida.] -- Compares bituminous coal, anthracite, and briquets.

Gives the results of tests and presents data for determining relative value of fuels for use in house heating boilers. EPA/ August STUDIES OF METHANOGENIC BACTERIA IN SLUDGE by P. Smith University of Florida Gainesville, Florida Grant No.

DJV Project Officer A. Venosa Wastewater Research Division Municipal Environmental Research Laboratory Cincinnati, Ohio MUNICIPAL ENVIRONMENTAL RESEARCH LABORATORY OFFICE OF RESEARCH AND. The methanogenic bacteria are unique among pro-karyotes because they produce a hydrocarbon, methane, as a major product of anaerobic metabolism.

This physiological property was proposed in by H. Barker as the main taxonomic characteristic of a morphologically diverse group of bacteria which he termed the by:   Metabolism Methane production.

Methanogens are known to produce methane from substrates such as H 2 /CO 2, acetate, formate, methanol and methylamines in a process called methanogenesis.

Different methanogenic reactions are catalyzed by unique sets of enzymes and reaction mechanism and energetics vary between one reaction and another, all of. This is in accordance with the study of Kiyohara et al. [21] where the activities of acidogenic and methanogenic bacteria fed with raw sludge were about and times higher under thermophilic.

The culture count for methane bacteria in sludge ranged between 10 5 and 10 8 per ml. The rate of fermentation and the efficiency of conversion of substrate into cell material were studied. Presence of an acetate-fermenting methanogenic organism was established but no pure cultures could be obtained.

Studies of methanogenic inhibition in the rumen confirm the toxicity of these compounds. a 50% loss of methanogenic activity was observed after exposing methanogenic sludge to mM laurate for only min. The methanogenic bacteria, p – In. isolate 2, 6, 7, 8 are methanogenic and 1, 3, 4, 5 are nonmethanogenic in nature.

The methane production of methanogenic and nonmethanogenic bacteria in consortia shows that methanogenic bacteria contribute more rather than nonmethanogenic bacteria. But when the studies were.

Finally, these intermediates are further converted to methane by methanogenic bacteria. In the absence of exogenous electron acceptors, the first step of the degradation of aromatic compounds in upflow anaerobic sludge blanket systems is an acetogenic process, which involves several microbial populations converting the aromatic compounds.

Finally, to study the feasibility of methanogenesis from CO 2 with elemental manganese added, test with CO 2 as sole carbon source was conducted. The anaerobic digestion system contained 50 ml synthetic medium and M CO 2. 1 g wet weight sludge (MLVSS of g/L) and 4 g /L elemental manganese were added into the system, respectively.

The. Visser's 18 research works with 1, citations and reads, including: Sulphide inhibition of methanogenic activity at various pH levels at 55°C. Guy D. Fauque, Larry L. Barton, in Advances in Microbial Physiology, Cytochromes from Methanogenic Bacteria. Methanogenic archaea constitute a phylogenetically diverse group of strictly anaerobic Euryarchaeota with an energy metabolism that is restricted to the production of methane from CO 2 + H 2, methanol, methylamines, formate, and/or acetate (Thauer, ).

Substrate competition between methanogenic and facultative bacteria under highly aerobic conditions was investigated in batch experiments. Natural mixed cultures of anaerobic bacteria immobilized in granular sludge were able to concurrently utilize oxygen and produce methane when supplied with ethanol as substrate.

Abstract. A study of the methane fermentation is unavoidably concerned with a study of microbial ecology because of the obligatory interactions between two major physiological participants, the chemoheterotrophic non-methanogenic bacteria and the methanogenic bacteria.

The inhibition of methanogenic activity test was carried out by pouring 48 ml of sludge into ml-capacity serum vials. Trying to simulate real conditions on an anaerobic digester, vials were filled with 80% of sludge from the anaerobic digester and 20% of loading sludge.

PDF | Molecular analysis of a low sulfate oil bearing environment was carried out using 16S rRNA gene sequencing technique and the effects of some | Find, read and cite all the research you. Read 8 answers by scientists with 6 recommendations from their colleagues to the question asked by Sadib Bin Kabir on Aug 7, Studies on nutrient requirements of one rumen strain (M1) and one sludge strain (PS) of Methanobacterium ruminantium and Methanobacterium strain MOH, isolated from Methanobacillus omelianskii, indicate that these bacteria, grown with H 2-CO 2 as the energy source, require NH 4 + as the main source of cell nitrogen and have little ability to incorporate or metabolize organic nitrogen.

Cappenberg TE. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. Field observations. Antonie Van Leeuwenhoek. ; 40 (2)– [Google Scholar] Edwards T, McBride BC. New method for the isolation and identification of methanogenic bacteria. Appl Microbiol.

Archer, D.B. Hydrogen‐using bacteria in a methanogenic acetate enrichment culture. Journal of Applied Bacteriology56, – In a study of the anaerobic utilization of acetate, an enrichmen. class. Some sulfate-reducing bacteria (SRB) are believed capable of degrading phenol (Boopathy ).

However, information related to the microbial characteristics of metha-nogenic sludge degrading phenol in wastewater is still very limited. In this study, microbial properties of a methanogenic .This study evaluated the effect of free ammonia on the methanogenic ability of the thermal hydrolysis and mesophilic combined process treating waste activated sludge (WAS) with conventional single.Kristijansson, J.

K., P. Schönheit, and R. K. Thauer. Different K s valuse for hydrogen of methanogenic bacteria and sulfate reducing bacteria: an explanation for the apparent inhibition of methanogenesis by sulfate. Arch. Microbiol. – Google Scholar.